SP1 antibody, IgG, Clone: Polyclonal, Unconjugated, Human

Catalog Number: GTX110593
Article Name: SP1 antibody, IgG, Clone: Polyclonal, Unconjugated, Human
Biozol Catalog Number: GTX110593
Supplier Catalog Number: GTX110593
Alternative Catalog Number: GTX110593-100,GTX110593-25
Manufacturer: GeneTex
Host: Human
Category: Antikörper
Application: ChIP, ICC, WB, IHC-P, IP
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein encompassing a sequence within the center region of human SP1. The exact sequence is proprietary.
Conjugation: Unconjugated
Alternative Names: Sp1 transcription factor
Clonality: Polyclonal
Concentration: 0.9 mg/ml (Please refer to the vial label for the specific concentration.)
Clone Designation: Polyclonal
Molecular Weight: 81
Isotype: IgG
NCBI: 6667
UniProt: P08047
Buffer: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Purity: Purified by antigen-affinity chromatography.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody (GTX110593) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SP1 antibody detects SP1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
SP1 stained by SP1 antibody (GTX110593) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using SP1(GTX110593) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
ChIP was performed with 293T chromatin extract and 5 μg of either normal rabbit IgG or anti-SP1 antibody. The precipitated DNA was detected by PCR with primer set targeting to MGARP promoter.
Immunoprecipitation of SP1 protein from THP-1 whole cell extracts using 5 μg of OCT1 antibody (GTX110593).
Western blot analysis was performed using OCT1 antibody (GTX110593) diluted at 1:250.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody (GTX110593) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using SP1(GTX110593) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
SP1 antibody detects SP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SP1 stained by SP1 antibody (GTX110593) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SP1 antibody detects SP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SP1 stained by SP1 antibody (GTX110593) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.