RUVBL1 antibody, IgG, Clone: Polyclonal, Unconjugated, Human

Catalog Number: GTX111294
Article Name: RUVBL1 antibody, IgG, Clone: Polyclonal, Unconjugated, Human
Biozol Catalog Number: GTX111294
Supplier Catalog Number: GTX111294
Alternative Catalog Number: GTX111294-100,GTX111294-25
Manufacturer: GeneTex
Host: Human
Category: Antikörper
Application: WB, IHC-P, ICC, IP
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein encompassing a sequence within the center region of human RUVBL1. The exact sequence is proprietary.
Conjugation: Unconjugated
Alternative Names: RuvB like AAA ATPase 1 , ECP-54 , ECP54 , INO80H , NMP 238 , NMP238 , PONTIN , Pontin52 , RVB1 , TIH1 , TIP49 , TIP49A
Clonality: Polyclonal
Concentration: 0.32 mg/ml (Please refer to the vial label for the specific concentration.)
Clone Designation: Polyclonal
Molecular Weight: 50
Isotype: IgG
NCBI: 8607
UniProt: Q9Y265
Buffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Purity: Purified by antigen-affinity chromatography.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (GTX111294) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with RUVBL1 antibody (GTX111294) diluted at 1:1000.
Immunoprecipitation of RUVBL protein from Jurkat whole cell extracts using 5 μg of RUVBL1 antibody (GTX111294).
Western blot analysis was performed using RUVBL1 antibody (GTX111294).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
RUVBL1 antibody detects RUVBL1 protein at cytoplasm in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
RUVBL1 antibody (GTX111294) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
RUVBL1 antibody detects RUVBL1 protein at cytoplasm and nucleus in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
RUVBL1 antibody (GTX111294) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
RUVBL1 antibody detects RUVBL1 protein at cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
RUVBL1 antibody (GTX111294) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
RUVBL1 antibody detects RUVBL1 protein at cytoplasm and nucleus in rat heart by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
RUVBL1 antibody (GTX111294) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
RUVBL1 antibody detects RUVBL1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RUVBL1 protein stained by RUVBL1 antibody (GTX111294) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RUVBL1(GTX111294) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min