Tyrosine Hydroxylase antibody, IgG, Clone: Polyclonal, Unconjugated, Human

Catalog Number: GTX113016
Article Name: Tyrosine Hydroxylase antibody, IgG, Clone: Polyclonal, Unconjugated, Human
Biozol Catalog Number: GTX113016
Supplier Catalog Number: GTX113016
Alternative Catalog Number: GTX113016-100,GTX113016-25
Manufacturer: GeneTex
Host: Human
Category: Antikörper
Application: WB, IHC-Fr, IHC-P, ICC
Species Reactivity: Mouse, Rodent, Human, Bovine, Rat
Immunogen: Recombinant protein encompassing a sequence within the center region of human Tyrosine Hydroxylase. The exact sequence is proprietary.
Conjugation: Unconjugated
Alternative Names: tyrosine hydroxylase , DYT14 , DYT5b , TYH
Clonality: Polyclonal
Concentration: 0.24 mg/ml (Please refer to the vial label for the specific concentration.)
Clone Designation: Polyclonal
Molecular Weight: 59
Isotype: IgG
NCBI: 7054
UniProt: P07101
Buffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Purity: Purified by antigen-affinity chromatography.
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Tyrosine Hydroxylase antibody (GTX113016) diluted at 1:2000.
Tyrosine Hydroxylase antibody detects Tyrosine Hydroxylase protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody (GTX113016) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Immunohistochemical analysis of Rat hindlimb pad skin tissue (paraformaldehyde-fixed frozen sections), using Tyrosine Hydroxylase(GTX113016) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Tyrosine Hydroxylase antibody detects TH protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
7.5% SDS-PAGE
Tyrosine Hydroxylase antibody (GTX113016) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of Rat hindlimb pad skin tissue (paraformaldehyde-fixed frozen sections), using Tyrosine Hydroxylase(GTX113016) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Tyrosine Hydroxylase antibody detects Tyrosine Hydroxylase protein at cytoplasm by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tyrosine Hydroxylase protein stained by Tyrosine Hydroxylase antibody (GTX113016) diluted at 1:400.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.
Blue: Hoechst 33342 staining.
Tyrosine Hydroxylase antibody detects Tyrosine Hydroxylase protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Tyrosine Hydroxylase antibody (GTX113016) diluted at 1:2500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Tyrosine Hydroxylase antibody detects Tyrosine Hydroxylase protein in midbrain dopaminergic neurons by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Green: Tyrosine Hydroxylase stained by Tyrosine Hydroxylase antibody (GTX113016) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Tyrosine Hydroxylase antibody detects TH protein by western blot analysis.
A. 30 μg NT2D1 whole cell lysate/extract
B. 30 μg PC-3 whole cell lysate/extract
C. 30 μg U87-MG whole cell lysate/extract
D. 30 μg SK-N-SH whole cell lysate/extract
7.5% SDS-PAGE
Tyrosine Hydroxylase antibody (GTX113016) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.