c-Kit (phospho Tyr730) antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: c-Kit (phospho Tyr730) antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX25633
• Supplier Catalog Number: GTX25633
• Alternative Catalog Number: GTX25633-50
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: IHC-P, WB
• Species Reactivity: Bovine, Canine, Gallus, Human, Mouse, Rat
• Immunogen: The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Kit that contains tyrosine 730. The sequence is conserved in mouse, rat and cow.
• Conjugation: Unconjugated
• Alternative Names: KIT proto-oncogene, receptor tyrosine kinase , C-Kit , CD117 , MASTC , PBT , SCFR

Product Description

This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known a

Product Properties

• Clonality: Polyclonal
• Concentration: Batch dependent (Please refer to the vial label for the specific concentration.)
• Clone Designation: Polyclonal
• Molecular Weight: 110
• Isotype: IgG
• NCBI: 3815
• UniProt: P10721
• Buffer: PBS pH7.3, 1mg/ml BSA, 50% Glycerol, 0.05% Sodium azide.
• Source: Human
• Purity: Purified by antigen-affinity chromatography
• Form: Liquid

Application Notes

• Application Notes: WB: 1:1000. IHC-P: 1:10-1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

WB analysis of 30 μg of A431 (Lane 1) treated for 10 minutes with 200 ng/ml of EGF (Lane 2), Rat Lung (Lane 3), HeLa (Lane 4), A549 (Lane 5) and serum starved A549 (Lane 6) lysate using GTX25633 c-Kit (phospho Tyr730) antibody.
Dilution : 1:1000

IHC-P analysis of human gastric carcinoma tissue using GTX25633 c-Kit (phospho Tyr730) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

GTX25633 WB Image