SARS-CoV-2 (COVID-19) Spike overexpression 293T whole cell lysate

Catalog Number: GTX535664
Article Name: SARS-CoV-2 (COVID-19) Spike overexpression 293T whole cell lysate
Biozol Catalog Number: GTX535664
Supplier Catalog Number: GTX535664
Alternative Catalog Number: GTX535664-200
Manufacturer: GeneTex
Category: Proteine/Peptide
Application: ELISA, WB
Species Reactivity: Virus
Alternative Names: 2019-nCoV Spike, COVID-19 Spike, SARS-CoV-2 Spike, covid-19, sars-cov-2
Whole-cell lysate of 293T cells overexpressing SARS-CoV-2 spike protein (full-length, no tag). The translated amino acid sequence is based on QHD43416.1. Lysate prepared in RIPA buffer with no reducing agents and is unboiled. Please see matched control 2
Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Buffer: 50 mM Tris-HCl (pH8.0), 150 mM NaCl, 0.5% sodium deoxycholate, 1% Triton X-100, 0.25% SDS, 1 mM PMSF, 5 µg/ml Leupeptin, 2 µg/ml Aprotinin, 1 µg/ml Pepstatin A, 0.025% ProClin 300.
Form: Liquid
SARS-CoV-2 (COVID-19) spike 293T overexpression cell lysates (GTX535664) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
GTX535664 WB Image
Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 spike (full length) transfected (GTX535664) 293T whole cell extracts using SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.