Prohibitin antibody [5H7], IgG1, Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: Prohibitin antibody [5H7], IgG1, Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: GTX60495
• Supplier Catalog Number: GTX60495
• Alternative Catalog Number: GTX60495-100
• Manufacturer: GeneTex
• Host: Mouse
• Category: Antikörper
• Application: ELISA, FACS, ICC, IHC-P, WB
• Species Reactivity: Human, Monkey, Mouse, Rat
• Immunogen: Purified recombinant fragment of human PHB expressed in E. Coli.
• Conjugation: Unconjugated
• Alternative Names: prohibitin , HEL-215 , HEL-S-54e , PHB1

Product Description

This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3 UTR, which is proposed to function as a trans-acting reg

Product Properties

• Clonality: Monoclonal
• Clone Designation: [5H7]
• Molecular Weight: 30
• Isotype: IgG1
• NCBI: 5245
• Pubmed: 30049713,
• UniProt: P35232
• Buffer: Ascites, 0.03% Sodium azide.
• Source: Human
• Purity: Unpurified
• Form: Liquid

Application Notes

• Application Notes: WB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX60495 IHC-P Image

FACS analysis of MCF-7 cells using GTX60495 Prohibitin antibody [5H7].
Blue : Prohibitin
Red : negative control

ICC/IF analysis of NIH3T3 cells using GTX60495 Prohibitin antibody [5H7].
Green : Prohibitin
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

IHC-P analysis of liver cancer tissue using GTX60495 Prohibitin antibody [5H7].

ELISA analysis of antigen using GTX60495 Prohibitin antibody [5H7].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

WB analysis of human Prohibitin (AA: 68-259) recombinant protein using GTX60495 Prohibitin antibody [5H7].

IHC-P analysis of stomach cancer tissue using GTX60495 Prohibitin antibody [5H7].

IHC-P analysis of lung cancer tissue using GTX60495 Prohibitin antibody [5H7].

WB analysis of A431 (1), MCF-7 (2), Jurkat (3), HeLa (4), HepG2 (5), A549 (6), NIH3T3 (7), Cos7 (8) and PC-12 (9) cell lysate using GTX60495 Prohibitin antibody [5H7].