SOX2 antibody [GT1352], IgG2a, Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: SOX2 antibody [GT1352], IgG2a, Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: GTX627405
• Supplier Catalog Number: GTX627405
• Alternative Catalog Number: GTX627405-100,GTX627405-25
• Manufacturer: GeneTex
• Host: Mouse
• Category: Antikörper
• Application: FACS, ICC, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse
• Immunogen: Recombinant protein encompassing a sequence within the center region of human SOX2. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: SRY-box 2 , ANOP3 , MCOPS3

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Clone Designation: [GT1352]
• Molecular Weight: 34
• Isotype: IgG2a
• NCBI: 6657
• UniProt: P48431
• Buffer: PBS, no Preservative.
• Purity: Affinity purified by Protein G.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. FACS: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX627405 IHC-P Image

SOX2 antibody [GT1352] detects SOX2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse E10.5 mouse embryo.
Green: Brachyury stained by Brachyury antibody (GTX133714) diluted at 1:250.
Red: SOX2, a nucleus marker, stained by SOX2 antibody [GT1352] (GTX627405) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Sox2 antibodies detects Sox2 proteins on embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE18.5).
Green: GFAP antibody (GTX108711) diluted at 1:1000.
Red: Sox2 antibody [GT1352] (GTX627405) diluted at 1:250.

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [GT1352] (GTX627405) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

SOX2 antibody [GT1352] detects SOX2 protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: SOX2 antibody [GT1352] dilution: 1:50

SOX2 antibody [GT1352] detects SOX2 protein by western blot analysis.
A. 30 μg human ESC whole cell lysate/extract
10% SDS-PAGE
SOX2 antibody [GT1352] (GTX627405) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

SOX2 antibody [GT1352] detects SOX2 protein by western blot analysis.
A. 30 μg mouse ESC whole cell lysate/extract
10% SDS-PAGE
SOX2 antibody [GT1352] (GTX627405) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [GT1352] (GTX627405) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

SOX2 antibody detects SOX2 protein at nucleus by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Red: SOX2 protein stained by SOX2 antibody (GTX627405) diluted at 1:200.
Green: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.