TDP43 antibody [GT225], IgG2b, Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: TDP43 antibody [GT225], IgG2b, Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: GTX630196
• Supplier Catalog Number: GTX630196
• Alternative Catalog Number: GTX630196-100,GTX630196-25
• Manufacturer: GeneTex
• Host: Mouse
• Category: Antikörper
• Application: IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein encompassing a sequence within the center region of human TDP43. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: TAR DNA binding protein , ALS10 , TDP-43

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Clone Designation: [GT225]
• Molecular Weight: 45
• Isotype: IgG2b
• NCBI: 23435
• UniProt: Q13148
• Buffer: PBS, no Preservative.
• Purity: Affinity purified by Protein G.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX630196 ICC/IF Image

TDP43 antibody [GT225] detects TDP43 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nestin stained by Nestin antibody (GTX133112) diluted at 1:500.
Red: TDP43 stained by TDP43 antibody [GT225] (GTX630196) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

TARDBP antibody [GT225] detects TARDBP protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
10 % SDS-PAGE
TARDBP antibody [GT225] (GTX630196) dilution: 1:1000

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TARDBP antibody [GT225] (GTX630196) diluted at 1:500.

TARDBP antibody [GT225] detects TARDBP protein by western blot analysis.
A. 30 μg BCL-1 whole cell lysate/extract
B. 30 μg Raw264.7 whole cell lysate/extract
10 % SDS-PAGE
TARDBP antibody [GT225] (GTX630196) dilution: 1:1000

TDP43 antibody [GT225] detects TDP43 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TDP43 antibody [GT225] (GTX630196) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

TDP43 antibody [GT225] detects TDP43 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TDP43 antibody [GT225] (GTX630196) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

TARDBP antibody [GT225] validation by siRNA knock-down.
Upperpanel: TARDBP antibody [GT225] GTX630196
Lower panel: GAPDH antibody (GTX100118)
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of TARDBP shRNA-transfected 293T cells
10 % SDS-PAGE
TARDBP antibody [GT225] (GTX630196) dilution: 1:3000
GAPDH antibody (GTX100118) dilution: 1:10000

TARDBP antibody [GT225] detects TARDBP protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
10 % SDS-PAGE
TARDBP antibody [GT225] (GTX630196) dilution: 1:1000