SDHA antibody [GT20710], IgG1, Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: SDHA antibody [GT20710], IgG1, Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: GTX632636
• Supplier Catalog Number: GTX632636
• Alternative Catalog Number: GTX632636-100,GTX632636-25
• Manufacturer: GeneTex
• Host: Mouse
• Category: Antikörper
• Application: ICC, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human SDHA. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: succinate dehydrogenase complex flavoprotein subunit A , CMD1GG , FP , PGL5 , SDH1 , SDH2 , SDHF

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Clone Designation: [GT20710]
• Molecular Weight: 73
• Isotype: IgG1
• NCBI: 6389
• UniProt: P31040
• Buffer: 1XPBS pH7, 20% Glycerol, no Preservative.
• Purity: Affinity purified by Protein G.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX632636 IHC-P Image

SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:3000.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:3000.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:3000.

HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:1000.

SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SDHA protein stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.