p53 antibody [DO1], IgG2a, Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: p53 antibody [DO1], IgG2a, Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: GTX70214
• Supplier Catalog Number: GTX70214
• Alternative Catalog Number: GTX70214-200
• Manufacturer: GeneTex
• Host: Mouse
• Category: Antikörper
• Application: ELISA, FACS, ICC, IHC-Fr, IHC-P, IP, WB
• Species Reactivity: Human, Mouse
• Immunogen: Recombinant human p53 expressed in E. coli.
• Conjugation: Unconjugated
• Alternative Names: tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53

Product Description

p53 antibody recognizes p53 protein, which has a predicted molecular weight of 44 kDa but is typically detected by western blot at 53 kDa. p53, known widely as a tumor suppressor, plays critical roles in various cellular processes including cell cycle re

Product Properties

• Clonality: Monoclonal
• Concentration: 0.25 mg/ml (Please refer to the vial label for the specific concentration.)
• Clone Designation: [DO1]
• Molecular Weight: 44
• Isotype: IgG2a
• NCBI: 7157
• Pubmed: 33061576, 27314325,
• UniProt: P04637
• Buffer: 1XPBS (pH7), 20% Glycerol, no Preservative.
• Source: Human
• Purity: Protein A purified
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:10000. IHC-P: 1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

p53 antibody [DO1] detects p53 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreatic cancer.
p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

p53 antibody [DO1] detects p53 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

p53 antibody [DO1] detects p53 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Immunoprecipitation of p53 protein from 293T whole cell extracts using 5 μg of p53 antibody [DO1] (GTX70214).
Western blot analysis was performed using p53 antibody [D01] (GTX70214).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:10000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) HCT116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX70214 WB Image