p53 antibody [DO1], IgG2a, Clone: Monoclonal, Unconjugated, Human

Catalog Number: GTX70214
Article Name: p53 antibody [DO1], IgG2a, Clone: Monoclonal, Unconjugated, Human
Biozol Catalog Number: GTX70214
Supplier Catalog Number: GTX70214
Alternative Catalog Number: GTX70214-200
Manufacturer: GeneTex
Host: Human
Category: Antikörper
Application: ELISA, IHC-Fr, FACS, ICC, WB, IHC-P, IP
Species Reactivity: Human, Mouse
Immunogen: Recombinant human p53 expressed in E. coli.
Conjugation: Unconjugated
Alternative Names: tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53
Clonality: Monoclonal
Concentration: 0.25 mg/ml (Please refer to the vial label for the specific concentration.)
Clone Designation: Monoclonal
Molecular Weight: 44
Isotype: IgG2a
NCBI: 7157
UniProt: P04637
Buffer: 1XPBS, 20% Glycerol (pH7)
Purity: Protein A purified
p53 antibody [DO1] detects p53 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreatic cancer.
p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
p53 antibody [DO1] detects p53 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
p53 antibody [DO1] detects p53 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Immunoprecipitation of p53 protein from 293T whole cell extracts using 5 μg of p53 antibody [DO1] (GTX70214).
Western blot analysis was performed using p53 antibody [D01] (GTX70214).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:10000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) HCT116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.