Caveolin 1 antibody [7C8], IgG2b, Unconjugated, Mouse, Monoclonal

Catalog Number: GTX79350
Article Name: Caveolin 1 antibody [7C8], IgG2b, Unconjugated, Mouse, Monoclonal
Biozol Catalog Number: GTX79350
Supplier Catalog Number: GTX79350
Alternative Catalog Number: GTX79350-100
Manufacturer: GeneTex
Host: Mouse
Category: Antikörper
Application: FACS, ICC, IHC-P, IP, WB
Species Reactivity: Hamster, Human, Mouse, Rat
Immunogen: Intercellular membrane protein-containing vesicles (containing GLUT4) from rat.
Conjugation: Unconjugated
Alternative Names: caveolin 1 , Cav
structural protein of lipid raft domains in the membrane called caveolae, functions as a cholesterol-binding/shuttling protein [RGD, Feb 2006]
Clonality: Monoclonal
Clone Designation: [7C8]
Isotype: IgG2b
NCBI: 25404
Pubmed: 28898551,
Buffer: Ascites, 0.05% Sodium azide.
Source: Rat
Purity: Unpurified
Form: Liquid
Application Notes: WB: 1:200-1:2000. ICC/IF: 1:125. IHC-P: 1:20. IP: 1 - 3 µl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
IHC-P analysis of rat lymph node tissue using GTX79350 Caveolin 1 antibody [7C8].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20
IHC-P analysis of rat lung tissue using GTX79350 Caveolin 1 antibody [7C8].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20
FACS analysis of L6 cells using GTX79350 Caveolin 1 antibody [7C8] compared to an isotype control (blue).
Dilution : 1:20 for 60 min at room temperature
Fixation : 2% paraformaldehyde
GTX79350 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX79350 Caveolin 1 antibody [7C8]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 in PBS for 15 minutes
Dilution : 1:100 for 1 hour at room temperature
FACS analysis of H-4-II-E cells using GTX79350 Caveolin 1 antibody [7C8] compared to an isotype control (blue).
Dilution : 1:20 for 60 min at room temperature
Fixation : 2% paraformaldehyde
ICC/IF analysis of cells using GTX79350 Caveolin 1 antibody [7C8].
WB analysis of 25 ug of A431 (Lane 1) and rat lung (Lane 2) cell lysates using GTX79350 Caveolin 1 antibody [7C8].
Dilution : 1:1000
FACS analysis of CHO cells using GTX79350 Caveolin 1 antibody [7C8] compared to an isotype control (blue).
Dilution : 1:20 for 60 min at room temperature
Fixation : 2% paraformaldehyde