Lamin A + C antibody [mab636], IgG2b, Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: Lamin A + C antibody [mab636], IgG2b, Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: GTX80813
• Supplier Catalog Number: GTX80813
• Alternative Catalog Number: GTX80813-200
• Manufacturer: GeneTex
• Host: Mouse
• Category: Antikörper
• Application: ICC, IHC-Fr, WB
• Species Reactivity: Bovine, Canine, Human, Mouse, Porcine
• Immunogen: Porcine lamin preparation.
• Conjugation: Unconjugated
• Alternative Names: lamin A/C , CDCD1 , CDDC , CMD1A , CMT2B1 , EMD2 , FPL , FPLD , FPLD2 , HGPS , IDC , LDP1 , LFP , LGMD1B , LMN1 , LMNC , LMNL1 , MADA , PRO1

Product Description

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disasse

Product Properties

• Clonality: Monoclonal
• Clone Designation: [mab636]
• Molecular Weight: 74
• Isotype: IgG2b
• NCBI: 4000
• UniProt: P02545
• Buffer: Tissue culture supernatant, 0.05% Sodium azide.
• Source: Pig
• Purity: Unpurified
• Form: Liquid

Application Notes

• Application Notes: WB: 1:100 - 1:2000. ICC/IF: 1:100. IHC-Fr: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

ICC/IF analysis of U2-OS cells (A) or HeLa cells (B) using GTX80813 Lamin A + C antibody [mab636].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:100 for at least 1 hour at room temperature

ICC/IF analysis of HeLa cells using GTX80813 Lamin A + C antibody [mab636].

WB analysis of various amounts of human fibroblast whole cell lysate using GTX80813 Lamin A + C antibody [mab636].
Dilution : 1:2000

ICC/IF analysis of A549 cells using GTX80813 Lamin A + C antibody [mab636].
Green : Primary antibody
Blue : Nuclei

ICC/IF analysis of MDCK cells using GTX80813 Lamin A + C antibody [mab636].
Red : Primary antibody
Blue : cell nucleus
Permeabilization : 0.1% Triton X-100
Dilution : 1:100 in blocking buffer for at least 1 hour at room temperature

ICC/IF analysis of HMVEC Cells using GTX80813 Lamin A + C antibody [mab636].
Green : Primary antibody
Blue : Nuclei

GTX80813 ICC/IF Image

WB analysis of 10μg nuclear extract from fractionated mouse tissue lysate using GTX80813 Lamin A + C antibody [mab636].
Dilution : 1:500

ICC/IF analysis of Human fibroblast cells using GTX80813 Lamin A + C antibody [mab636].
Fixation : methanol
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:200 for at least 1 hour at room temperature