Thyroid Hormone Receptor alpha-2 antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Thyroid Hormone Receptor alpha-2 antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX82858
• Supplier Catalog Number: GTX82858
• Alternative Catalog Number: GTX82858-200
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic Peptide: S(455) R A V C G E D D S S E A S S(469)
• Conjugation: Unconjugated
• Alternative Names: thyroid hormone receptor alpha , 6430529J03Rik , AW259572 , Erba , Nr1a1 , Rvr , T3R[a] , T3Ralpha , Thra1 , Thra2 , c-erbA-1 , c-erbA-alpha , c-erbAalpha

Product Description

The protein encoded by this gene is one of several nuclear hormone receptors that bind thyroid hormones such as triiodothyronine and thyroxine with high affinity. The encoded protein is a transcription factor that can activate or repress transcription. S

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Clone Designation: Polyclonal
• Molecular Weight: 55
• Isotype: IgG
• NCBI: 21833
• UniProt: P63058
• Buffer: PBS, 1mg/ml BSA, 0.05% Sodium azide.
• Source: Mouse
• Purity: Purified by antigen-affinity chromatography
• Form: Liquid

Application Notes

• Application Notes: WB: 1:100. ICC/IF: 1:10-1:200. IHC-P: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

WB analysis of 25 ug of mouse thyroid (Lane 1), NIH-3T3 (Lane 2) and A431 (Lane 3) cell lysates using GTX82858 Thyroid Hormone Receptor alpha-2 antibody.
Dilution : 1:100

IHC-P analysis of human skin tissue using GTX82858 Thyroid Hormone Receptor alpha-2 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

IHC-P analysis of human thyroid tissue using GTX82858 Thyroid Hormone Receptor alpha-2 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:200

GTX82858 ICC/IF Image

IHC-P analysis of mouse thyroid tissue using GTX82858 Thyroid Hormone Receptor alpha-2 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

ICC/IF analysis of A431 cells using GTX82858 Thyroid Hormone Receptor alpha-2 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

IHC-P analysis of human thyroid tissue using GTX82858 Thyroid Hormone Receptor alpha-2 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

ICC/IF analysis of NIH-3T3 cells using GTX82858 Thyroid Hormone Receptor alpha-2 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

IHC-P analysis of mouse thyroid tissue using GTX82858 Thyroid Hormone Receptor alpha-2 antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min
Dilution : 1:200