Profilin 1 antibody [2D2], IgG1, Unconjugated, Mouse, Monoclonal

Catalog Number: GTX83902
Article Name: Profilin 1 antibody [2D2], IgG1, Unconjugated, Mouse, Monoclonal
Biozol Catalog Number: GTX83902
Supplier Catalog Number: GTX83902
Alternative Catalog Number: GTX83902-100
Manufacturer: GeneTex
Host: Mouse
Category: Antikörper
Application: FACS, ICC, IHC-P, WB
Species Reactivity: Canine, Human, Monkey, Rat
Immunogen: Full length human recombinant protein of human PFN1 (NP_005013) produced in HEK293T cell.
Conjugation: Unconjugated
Alternative Names: profilin 1 , ALS18
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.
Clonality: Monoclonal
Concentration: 0.58 mg/ml (Please refer to the vial label for the specific concentration.)
Clone Designation: [2D2]
Molecular Weight: 15
Isotype: IgG1
NCBI: 5216
UniProt: P07737
Buffer: PBS pH7.3, 1% BSA, 50% Glycerol, 0.02% Sodium azide.
Source: Human
Purity: Purified by affinity chromatography
Form: Liquid
Application Notes: WB: 1:2000. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
IHC-P analysis of human pancreas tissue using GTX83902 Profilin 1 antibody [2D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
WB analysis of various cell lines using GTX83902 Profilin 1 antibody [2D2].
Loading : 35 ug per lane
FACS analysis of Jurkat cells using GTX83902 Profilin 1 antibody [2D2].
Red : Primary antibody
Blue : Negative control antibody
IHC-P analysis of human kidney tissue using GTX83902 Profilin 1 antibody [2D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
IHC-P analysis of human prostate tissue using GTX83902 Profilin 1 antibody [2D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50
FACS analysis of HeLa cells using GTX83902 Profilin 1 antibody [2D2].
Red : Primary antibody
Blue : Negative control antibody
GTX83902 FACS Image
WB analysis of HEK293T cells transfected with Profilin 1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83902 Profilin 1 antibody [2D2].
Loading : 5 ug per lane
ICC/IF analysis of COS7 cells transiently transfected with Profilin 1 plasmid using GTX83902 Profilin 1 antibody [2D2].