ATM phospho S1981 Antibody, IgG2a, Clone: [7C10D8], Mouse, Monoclonal

Product Overview

• Article Name: ATM phospho S1981 Antibody, IgG2a, Clone: [7C10D8], Mouse, Monoclonal
• Biozol Catalog Number: ROC-200-301-500
• Supplier Catalog Number: 200-301-500
• Alternative Catalog Number: ROC-200-301-500
• Manufacturer: Rockland Immunochemicals
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IHC
• Species Reactivity: Human, Mouse
• Immunogen: This antibody was produced from a synthetic peptide S-L-A-F-E-E-G-Sp-Q-S-T-T-I-S-S corresponding to aa 1974-1988 of human ATM.
• Conjugation: Unconjugated
• Alternative Names: mouse anti-ATM antibody, mouse anti-ATMpS1981 antibody, mouse anti- ATM pS1981 antibody, DKFZp781A0353 antibody, Human phosphatidylinositol 3 kinase homolog antibody, MGC74674 antibody, Serine protein kinase ATM antibody, T cell prolymphocytic leukemia antibody

Product Properties

• Clonality: Monoclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Clone Designation: [7C10D8]
• Isotype: IgG2a
• NCBI: 472
• UniProt: Q13315
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, Flow Cytometry: User Optimized, IHC: 1:100 - 1:500, WB: 1:500- 1:2,000
• Application Notes: Anti-ATM Protein Kinase pS1981 (MOUSE) Monoclonal Antibody clone has been tested by ELISA and optimized for IHC, but may also be suitable for Western blot, flow cytometry, immunoprecipitation, and immunofluorescence microscopy. Indirect immunoperoxidase

Images

ATM authophosphorylation.

Anti ATM monoclonal Antibody was used to show constitutive activation of the ATM pathway in human urinary bladder cancer. Anti-ATM Protein Kinase pS1981 (clone 7C10D8 ) was used to stain normal uroepithelium, early superficial lesions (Ta), and earliest invasive (T1) primary carcinomas. ATM protein is ubiquitously expressed, but Ser 1981-phosphorylated ATM (pS-ATM) is detectable only in tumor tissues. See Bartkova et al. for additional details. Reprinted by permission from Nature (Cell Cycle 4,(6), 2005) Macmillan Publishers Ltd.

Determination of ATM and ATMp antibodies specificity by western blotting. Western blot analysis of neuroretinas and brains from adult Atm-deficient and adult C57BL/6J mice was performed with monoclonal antibodies raised against ATM and ATMp, and with polyclonal antibody beta-actin (internal control). Specific bands for ATM (350kDa), ATMp (350kDa), and beta-actin (35kDa) were detected by western blot analysis of protein homogenates extracted from different mouse tissues (neuroretina and brain). No band could be detected in protein homogenates from Atm-deficient mice tissues blotted with either specific ATM or specific ATMp antibody. Figure provided by CiteAb. Source: Mol Vis, PMID: 19234633.