Leucocytes isolated from porcine gut lamina propria.
Recognizes a 55kD porcine homologue to the human CD4 antigen found on the surface of helper-T cells. MIL-17 was confirmed as a member of the CD4a cluster at the 'Third International Workshop on Swine Leukocyte Differentiation Antigens (Haverson et al. 2001). Porcine CD4 is a type 1 trans-membrane member of the immunoglobulin superfamily. Pigs appear unusual amongst mammalian species as they appear to have four populations of resting T lymphocytes. In addition to the two populations of mutually exclusive CD4+/CD8- and CD4-/CD8+ lymphocytes, they also appear to have significant populations of CD4-/CD8- and CD4+/CD8+ cells. Lymphoblasts with a double positive phenotype have been described in other species but this is not the case for mature T lymphocytic calls (Saalmuller et al. 1987) Stains a population of cells with characteristic lymphocyte morphology in immunohistochemistry (Inman et al. 2010). Applications: Suitable for use in Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Immunohistochemistry: The epitope recognized is reported to be sensitive to formaldehyde fixation and tissue processing. Acetone fixation is recommeneded for frozen sections. Paraffin sections are not recommeneded. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Clonality:
Monoclonal
Clone Designation:
[MIL17]
Isotype:
IgG2b
Purity:
Purified by Protein A affinity chromatography from tissue culture supernatant.
Form:
Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
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