GREM2, also known as PRDC, is one of several BMP antagonists that regulate the functions of BMPs during embryonic development and tissue differentiation. BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. The PRDC gene is mapped to chromosome 1q43. Sample Type: Cell culture supernates, serum, plasma (Heparin or EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Human GREM2/Prdc. Sensitivity: <12pg/ml Range: 62.5pg/ml-4000pg/ml Specificity: Recognizes natural and recombinant monkey GREM2/Prdc. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Monkey GREM2/Prdc BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A specific for Monkey GREM2/Prdc has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: R22-Q168) and test samples are added to the wells, a biotinylated detection specific for Monkey GREM2/Prdc is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Monkey GREM2/Prdc amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Monkey GREM2 antibody, 1x plate Lyophilized recombinant Monkey GREM2 standard, 2x10ng/tube Biotinylated anti-Monkey GREM2 antibody, 1x130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100) Sample diluent buffer, 1x30ml Antibody diluent buffer, 1x12ml ABC diluent buffer, 1x12ml TMB color developing agent, 1x10ml TMB stop solution, 1x10ml PBS washing buffer Powder for 1000ml, 1x buffer Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
