Phosphorylation of endogenous proteins is a crucial mechanism in cell signaling pathways. Still the availability of validated antibody pairs and optimized ELISA protocol often presents a critical limitation for cell signaling research.
Expedeon's new ELISA-One™ format are ready-to-use ELISA kits for the detection of endogenous proteins that undergo phosphorylation. ELISA-ONE™ is a sandwich ELISA based on the CaptSure™ technology. The detection of the analyte (in its phosphorylated or unphosphorylated status, or both) is done in one incubation step with an HRP-labeled antibody.
Available targets: AKT1/2/3, ERK 1/2, NF-κB, SMAD1, STAT3, and STAT5 (each phosphorylated & total).