Expedeon ELISA-One™

Easy detection of phosphorylated proteins

Phosphorylation of endogenous proteins is a crucial mechanism in cell signaling pathways. Still the availability of validated antibody pairs and optimized ELISA protocol often presents a critical limitation for cell signaling research.

Expedeon's new ELISA-One™ format are ready-to-use ELISA kits for the detection of endogenous proteins that undergo phosphorylation. ELISA-ONE™ is a sandwich ELISA based on the CaptSure™ technology. The detection of the analyte (in its phosphorylated or unphosphorylated status, or both) is done in one incubation step with an HRP-labeled antibody.

Available targets: AKT1/2/3, ERK 1/2, NF-κB, SMAD1, STAT3, and STAT5 (each phosphorylated & total).


  • One incubation step (ab pair + cell lysate)
  • Cell lysis & analysis can be performed in the same well
  • Validated antibody pair & Optimized protocol
  • Proved performance with endogenous proteins
  • Flexible plate format (12x8 strip well)

>> ELISA-One™ in our BIOZOL web shop


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