iLite Insulin*

Artikelnummer: EUD-BM3060
Artikelname: iLite Insulin*
Artikelnummer: EUD-BM3060
Hersteller Artikelnummer: BM3060
Alternativnummer: EUD-BM3060
Hersteller: Svar Life Science
Kategorie: Zellen/Zellkultur

This iLite® insulin assay offers robust, quantitative measurement of insulin receptor activation, facilitating characterization of insulin analogs and biosimilars.

  • High sensitivity and large dynamic range
  • "Assay-ready" format for rapid and convenient use
  • Reproducible and biologically relevant results
  • Reduced assay variability and higher reproducibility compared to cells in culture
  • Normalization of cell counts and serum matrix effects using a second reporter gene

 

Product Overview

The insulin-responsive iLite® Insulin Assay Ready Cells provide the basis for the establishment of a rapid, sensitive, and highly specific assay for the quantification of insulin activity that can be completed within 6 hours.

iLite Insulin assay ready cells are avian DT-40 cells (ATCC#CRL211) which has been genetically engineered to be responsive to Insulin, resulting in the specific expression of Firefly Luciferase. Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under control of a constitutive promotor.

The assay can be used to quantify the activity of insulin or an insulin analog in human serum without interference from factors present in human serum, including IGF-1. In addition, iLite assay ready cells can be used for measuring the presence of neutralizing anti-insulin antibodies.

The ATCC® CRL-2111 cell line is used under a license from ATCC®

 

 

What's in the box?

>250 µl of Assay Ready Cells suspended in cryoprotective medium. 

 

Specifications

Product code BM3060
Assay target Insulin
Size 1 vial corresponding to one 96 well plate
Technology Cell-based reporter gene assay
Detection system Luminescence
Regulatory status Research Use Only
Assay time 5.5-6h
Formulierung: Assay Ready Cells
Insulin