iLite GM-CSF*

Produktübersicht

• Artikelname: iLite GM-CSF*
• Artikelnummer: EUD-BM4050
• Hersteller Artikelnummer: BM4050
• Alternativnummer: EUD-BM4050
• Hersteller: Svar Life Science
• Kategorie: Zellen/Zellkultur

Zusatzinformationen

The iLite® GM-CSF assay enables functional quantification of granulocyte-macrophage colony-stimulating factor activity. Ideal for potency testing of cytokine-based biologics.

• High sensitivity and large dynamic range
• "Assay-ready" format for rapid and convenient use
• Reproducible and biologically relevant results
• Reduced assay variability and higher reproducibility compared to cells in culture
• Normalization of cell counts and serum matrix effects using a second reporter gene

 

Product Overview

iLite® GM-CSF Assay Ready Cells are a genetically engineered reporter gene cell line (U937) responsive to granulocyte-macrophage colony-stimulating factor (GM-CSF) by specific and proportional expression of Firefly Luciferase.

They can be used for the quantification GM-CSF activity, GM-CSF inhibitor activity, and for determination of neutralizing antibodies against such either in buffer systems or human serum.

As a functional assay, iLite GM-CSF assay ready cells can be used to determine the activity of GM-CSF, or of GM-CSF inhibitors, as well as neutralizing antibodies, all using the same assay set-up.

The assay ready iLite GM-CSF cells  Normalization of cell counts and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under the control of a constitutive promotor.

 

What's in the box?

 >250 µL of Assay Ready Cells suspended in cryoprotective medium.

 

Specifications

• Product code: BM4050
• Assay target: GM-CSF
• Size: 1 vial corresponding to one 96 well plate
• Technology: Cell-based reporter gene assay
• Detection system: Luminescence
• Regulatory status: Research Use Only

 

Produkteigenschaften

• Formulierung: Assay Ready Cells

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