HIF1 alpha antibody, Unconjugated, Rabbit, Polyclonal

Artikelname:	HIF1 alpha antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer:	GTX127309
Hersteller Artikelnummer:	GTX127309
Alternativnummer:	GTX127309-100,GTX127309-25
Hersteller:	GeneTex
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ChIP, ICC, IHC-Fr, IHC-P, IP, WB
Spezies Reaktivität:	Bovine, Human, Mouse, Rabbit, Rat
Immunogen:	Recombinant protein encompassing a sequence within the C-terminus region of human HIF1 alpha. The exact sequence is proprietary.
Konjugation:	Unconjugated
Alternative Synonym:	hypoxia inducible factor 1 subunit alpha , HIF-1-alpha , HIF-1A , HIF-1alpha , HIF1 , HIF1-ALPHA , MOP1 , PASD8 , bHLHe78

HIF1 alpha antibody recognizes HIF1 alpha (HIF1a) protein, which is a subunit of the heterodimeric transcription factor hypoxia-inducible factor-1 (HIF-1). HIF-1 is a master regulator of homeostatic adaption to hypoxia (low oxygen supply) in various phys

Klonalität:	Polyclonal
Konzentration:	1.44 mg/ml (Please refer to the vial label for the specific concentration.)
Molekulargewicht:	93
Sensitivitaet:	Knockdown/Knockout validation was supported by references (PMID:25428606)
NCBI:	3091
UniProt:	Q16665
Puffer:	1XPBS pH7, 20% Glycerol, 0.025% ProClin 300.
Reinheit:	Purified by antigen-affinity chromatography.
Formulierung:	Liquid

Anwendungsbeschreibung:

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:570. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

	GTX127309 WB Image
	HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis. Sample: Hela cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIF1 alpha stained by HIF1 alpha antibody (GTX127309) diluted at 1:500.
	Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	Immunoprecipitation of HIF1 alpha protein from HepG2 whole cell extracts treated with 500 μM CoCl2 for 24 hr using 5 μg of HIF1 alpha antibody (GTX127309). Western blot analysis was performed using HIF1 alpha antibody (GTX127309) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
	ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-HIF1 alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to VEGF promoter.
	HIF1 alpha antibody detects HIF1 alpha protein at nucleus on human kidney cancer by immunohistochemical analysis. Sample: Paraffin-embedded human kidney cancer. HIF1 alpha antibody (GTX127309) dilution: 1:500.
	HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis. Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIF1 alpha protein stained by HIF1 alpha antibody (GTX127309) diluted at 1:200. Blue: Hoechst 33342 staining.
	HIF1 alpha antibody detects HIF1 alpha protein at nucleus in CoCl2-treated samples by confocal immunofluorescent analysis. Samples: Untreated (left panel) and 500μM CoCl2 treated (right panel) HeLa cells for 24 hrs were fixed in 4% paraformaldehyde for 15 min Green: HIF1alpha protein stained by HIF1 alpha antibody (GTX127309) diluted at 1:1000 Red: Alpha-tubulin, a cytoskeleton marker, stained by Alpha-tubulin antibody (GTX11304) diluted at 1:2500 Blue: Hoechst 33342 staining. Scale bar = 10 μm. Images captured by Olympus FV10i Confocal Laser Scanning Microscope
	HIF1 alpha antibody detects HIF1 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
	HIF1 alpha antibody detects HIF1 alpha protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HIF1 alpha stained by HIF1 alpha antibody (GTX127309) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 μm.
	Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF1 alpha antibody (GTX127309) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.