Description
DyLight 488 Horse Anti-Rabbit IgG Antibody can be used for tissue staining and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.
Features:
- Affinity-purified, ultrapure, high affinity antibody
- Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species
- Recognizes both heavy and light chains (H+L)
- Optimally labeled with DyLight™ 488 to provide the brightest label for fluorescence microscopy
- Supplied in solution
- Excitation: 493 nm
- Emission: 518 nm
- Color: Green
Specifications
| Unit Size |
1.5 mg |
| Applications |
Immunofluorescence, In situ hybridization, Blotting Applications, Flow Cytometry/Cell Separation |
| Concentration |
1.5 mg active conjugate/ml |
| Recommended Storage |
2-8 °C |
| Solution |
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. |
| Maximum Emission |
518 nm |
| Maximum Excitation |
493 nm |
| Recommended Usage |
Recommended concentration range for use 5-20 µg/ml. If this antibody is to be used in tissues which may contain cross-reacting endogenous immunoglobulins, dilution of this antibody may be made in buffers containing 2% normal serum from the same species as the tissue. |
| Target Species |
Rabbit |
| Conjugate |
DyLight 488 |
| Color of Fluorescence |
Green |
| Host Species |
Horse |
| Format |
Concentrate |
