5-Ethynyl-UTP (5-EUTP), CAS [[1193451-16-9 sodium salt 1004955-96-7 free acid]]

Produktübersicht

• Artikelname: 5-Ethynyl-UTP (5-EUTP), CAS [[1193451-16-9 sodium salt 1004955-96-7 free acid]]
• Artikelnummer: BCL-BCT-07-L
• Hersteller Artikelnummer: BCT-07-L
• Alternativnummer: BCL-BCT-07-L
• Hersteller: baseclick
• Kategorie: Biochemikalien
• Alternative Synonym: Modified Triphosphate for labeling of 3END RNA or ssDNA

Produktbeschreibung

5-Ethynyl-UTP (5-EUTP) can be incorporated into RNA using standard nucleotide mixtures and T7 RNA polymerase in vitro transcription. For a partial modified RNA we recommend to replace the natural base by only 1-10%. Modified mRNA is ready for labeling with marker azides (e.g. fluorescent dyes for tracking and imaging or cross-linking to other biomolecules) by click chemistry.

Produkteigenschaften

• Konzentration: 100 mM
• Molekulargewicht: 507.97 g/mol
• Reinheit: 98% (HPLC)
• Formulierung: Liquid
• CAS Nummer: [1193451-16-9 sodium salt 1004955-96-7 free acid]
• Formel: C11H15N2O15P3

Anwendungsbeschreibung

• Anwendungsbeschreibung: During in vitro transcription, RNA polymerases efficiently incorporate 5-EUTP into RNA, introducing reactive alkyne group that enables selective post transcriptional tagging using bioorthogonal click chemistry, particularly copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC).

Bilder

5-Ethynyl-UTP (5-EUTP)

5-Ethynyl-UTP (5-EUTP)