Produktbeschreibung
The ZV-67 monoclonal antibody reacts with Zika virus envelope (E) protein at the lateral ridge of domain III (DIII-LR), and it is reported to neutralize infections of American, African, and Asian strains of Zika virus (ZIKV). Biologically, ZIKV is a positive-stranded RNA virus that is transmitted through Aedes aegypti (the yellow fever mosquito). ZIKV gets transmitted through sexual contact as well, and ZIKV infections during pregnancy have been linked to developmental defects in newborns e.g., congenital zika syndrome (CZS), which is characterized by neuropathogenesis and microcephaly in unborn babies, eye abnormalities, arthrogryposis, etc., In adults also, the ZIKV infections lead to serious neurological complications. ZIKVs genome for seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5) and the ZIKV enveloped virion consists of three structural proteins - capsid (C), membrane (M), and envelope (E). In experimental studies, the E protein of ZIKV is often targeted with the ZV-67 monoclonal antibody which was generated by immunizing a lethal mouse model with ZIKV (MR-766), ZIKV (H/PF/2013), and the recombinant E protein DIII domain. This antibody specifically binds to the immunoglobulin-like segment DIII lateral ridge (DIII-LR) of the ZIKV E protein, which is responsible for viral assembly, attachment, entry, and fusion. In ELISA and Western blot, the ZV-67 monoclonal antibody has been reported to specifically detect the recombinant ZIKV E protein DIII and ZIKV E protein reporter virus particles (RVPs) in transfected cells. Several in vitro investigations have reported the neutralization potential of the ZV-67 monoclonal antibody against ZIKV H/PF/2013, ZIKV/KOR/SNU/2016, ZIKV/PRVABC59, African strains (MR-766, Uganda 1947, and Dakar 41519, Senegal 1982), American stain (Paraiba 2015, Brazil strain), Zika virus MR766 mutants, and ZIKV E protein-RVPs. In animal studies, in vivo administration of the ZV-67 monoclonal antibody provided significant protection against ZIKV Dakar 41519 infection in young mice and against ZIKV Asian strain GZ01 infection in pregnant mice. The ZV-67 monoclonal antibody does not show any cross-reactivity with Japanese encephalitis (JEV) or Dengue virus (DENV).
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