Integrin Alpha 3 + Beta 1 Rabbit Polyclonal Antibody, Unconjugated

Produktübersicht

• Artikelname: Integrin Alpha 3 + Beta 1 Rabbit Polyclonal Antibody, Unconjugated
• Artikelnummer: BYT-ORB10925
• Hersteller Artikelnummer: orb10925
• Alternativnummer: BYT-ORB10925-100,BYT-ORB10925-200,BYT-ORB10925-50
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FC, IF, IHC-Fr, IHC-P, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human Integrin Alpha 3(976-1025aa) + Beta 1(35-47aa)
• Konjugation: Unconjugated
• Alternative Synonym: Integrin alpha 3/beta 1, Integrin VLA 4 beta subunit, alpha 3 subunit of VLA 3 receptor, CD29, CD49C, Fibronectin receptor, Fibronectin receptor beta subunit, Galactoprotein B3, GAPB3, VL3A, VLA 3 alpha chain, VLA3a.

Produktbeschreibung

Integrin Alpha 3 + Beta 1 Rabbit Polyclonal Antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1mg/ml
• Molekulargewicht: 115 kDa
• UniProt: P26006
• Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Formulierung: Liquid
• Target-Kategorie: ITGA3

Anwendungsbeschreibung

• Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test

Bilder

Sample: Lung (Mouse) Lysate at 30 ug, Primary: Anti-Integrin Alpha 3 + Beta 1 (orb10925) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 115 kD, Observed band size: 130 kD.

Blank control: A431. Primary Antibody (green line): Rabbit Anti-Integrin Alpha 3 + Beta 1 antibody (orb10925), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Integrin Alpha 3 + Beta 1) Polyclonal Antibody, Unconjugated (orb10925) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Cerebrum (Mouse) Lysate at 30 ug, Primary: Anti-Integrin Alpha 3 + Beta 1 (orb10925) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 115 kD, Observed band size: 117 kD.