SARS-CoV-2 (COVID-19) Spike S2 Single Domain Antibody [P1G5], Unconjugated, Camelus, Recombinant Preis auf Anfrage

Produktübersicht

• Artikelname: SARS-CoV-2 (COVID-19) Spike S2 Single Domain Antibody [P1G5], Unconjugated, Camelus, Recombinant Preis auf Anfrage
• Artikelnummer: BYT-ORB1274188
• Hersteller Artikelnummer: orb1274188
• Alternativnummer: BYT-ORB1274188-0.02,BYT-ORB1274188-0.1
• Hersteller: Biorbyt
• Wirt: Camelus
• Kategorie: Antikörper
• Applikation: ELISA
• Spezies Reaktivität: Virus
• Immunogen: SARS-CoV-2 Spike S2 protein containing C-terminal His Tag. The protein was expressed in human 293 cells (HEK293). It contains amino acids Ser 686 - Pro 1213.
• Konjugation: Unconjugated
• Alternative Synonym: SARS-CoV-2 (COVID-19) Spike S2 Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Spike protein, Surface Glycoprotein, covid-19, sars-cov-2

Produktbeschreibung

SARS-CoV-2 (COVID-19) Spike S2 Single Domain Antibody [P1G5]

Produkteigenschaften

• Klonalität: Recombinant
• Konzentration: batch dependent
• Klon-Bezeichnung: [P1G5]
• NCBI: 43416
• Puffer: SARS-CoV-2 (COVID-19) Spike S2 Antibody is supplied in PBS.
• Formulierung: Liquid
• Target-Kategorie: S

Bilder

ELISA Validation with Spike Trimer Proteins of SARS-CoV-2 Variants and SARS-CoV-1 Antibodies: SARS-CoV-2 (COVID-19) Spike S2 Antibody, orb1274188. A direct ELISA was performed using Spike trimer protein of SARS-CoV-2 Variants (Delta and Omicron) and SARS-CoV-1 as coating antigens at 1 µg/mL and the anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (orb1274188) as the capture antibody, following by anti-cMyc-tag antibody (orb1239964) at 1 µg/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 16 ng/mL to 2000 ng/mL.

Epitope Mapping of four Spike S2 Antibodies by Direct Competitive ELISA A direct competitive ELISA was developed for the epitope mapping using Spike trimer proteins of SARS-CoV-2 Delta variant at 1 µg/ml as the coating antigen and biotinylated Spike S2 single domain antibodies (sdAbs) as the competitive antibodies. The sdAb mixtures (biotinylated and unconjugated sdAbs) were added to ELISA plate and streptavidin-HRP was used to detect the captured biotinylated sdAbs. The assay suggests the antibodies bind to different epitopes of the Spike trimer.