Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions. The variable region was obtained from a mouse immunized with purified recombinant SARS-CoV-2 Spike S1 Protein. Pseudovirus assay shows that this antibody has potent neutralizing activity against pseudovirus bearing SARS-CoV-2 Spike protein.
Lyophilized from 0.22 um filtered solution in PBS, pH 7.4 . Normally trehalose is added as protectant before lyophilization. Contact us for customized product form or formulation.
Formulierung:
Lyophilized
Target-Kategorie:
S
Anwendungsbeschreibung:
Application Notes: This antibody can be paired with other Anti-SARS-CoV-2 Spike S1 antibodies to detect SARS-CoV-2 Spike S1 protein in sandwich ELISA or LFA assay
Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized SARS-CoV-2 S1 protein, His Tag at 2 ug/mL (100 uL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 with a linear range of 0.2-3 ng/mL (QC tested).
Detection SARS-CoV-2 S protein RBD by Sandwich ELISA Assay. Immobilized Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 at 2 ug/mL (100 uL/well) can bind S protein RBD. And then add Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 at 1:5000. Detection was performed using HRP-conjugated streptavidin with sensitivity of 25 pg/mL (Routinely tested).
Immobilized Human ACE2, His Tag at 2 ug/mL (100 uL/well) can bind pre-mixed increasing concentrations of Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 and 0.4 ug/mL (50 uL/well) Biotinylated S protein RBD, His,Avitag with a half maximal inhibitory concentration (IC50) of 0.33 ug/mL (Routinely tested).
Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind SARS-CoV-2 S protein RBD, His Tag with an affinity constant of 0.375 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
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