CD3E Antibody, IgG2b, Unconjugated, Mouse, Monoclonal

Produktübersicht

• Artikelname: CD3E Antibody, IgG2b, Unconjugated, Mouse, Monoclonal
• Artikelnummer: BYT-ORB1333884
• Hersteller Artikelnummer: orb1333884
• Alternativnummer: BYT-ORB1333884-100
• Hersteller: Biorbyt
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: IF, IHC, WB
• Spezies Reaktivität: Canine, Human, Monkey
• Immunogen: Full length human recombinant protein of human CD3E(NP_000724) produced in HEK293T cell.
• Konjugation: Unconjugated
• Alternative Synonym: IMD18, T3E, TCRE

Produktbeschreibung

CD3E Antibody

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [OTI3E10]
• Molekulargewicht: 20.7 kDa
• Isotyp: IgG2b
• NCBI: 000733
• UniProt: P07766
• Puffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Target-Kategorie: CD3E

Anwendungsbeschreibung

• Application Verdünnung: WB 1:200~4000, IHC 1:150, IF 1:100
• Anwendungsbeschreibung: Application Notes: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process

Bilder

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD3E (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD3E. Positive ly

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD3E monoclonal antibody (HepG2: human, HeLa: human, SVT2: mouse, A549: human, COS7: monkey, Jurkat: human, MDCK: canine, PC12: rat, MCF7: human).

Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-CD3E monoclonal antibody at 1:200 (1: Testis, 2: Uterus, 3: Breast, 4: Brain, 5: Liver, 6: Ovary, 7: Thyroid gland, 8: Colon).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3min) (1:2000) (1:4000)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3min) (1:2000) (1:4000)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3min) (1:2000) (1:4000)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120C for 3min) (1:2000) (1:4000)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Anti-CD3E mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD3E.