Rabbit anti-P-Selectin/CD62P Recombinant Monoclonal Antibody, Clone: [BLR267L], Unconjugated

Produktübersicht

• Artikelname: Rabbit anti-P-Selectin/CD62P Recombinant Monoclonal Antibody, Clone: [BLR267L], Unconjugated
• Artikelnummer: BYT-ORB1784576
• Hersteller Artikelnummer: orb1784576
• Alternativnummer: BYT-ORB1784576-100
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ICC, IF, IHC, WB
• Spezies Reaktivität: Human
• Immunogen: Residues 42-771 (ECD)
• Konjugation: Unconjugated
• Alternative Synonym: LECAM3, leukocyte-endothelial cell adhesion molecule 3, PADGEM, platelet activation dependent granule-external membrane protein, platelet alpha-granule membrane protein, P-selectin, GRMP, PSEL, granule membrane protein 140kDa, granule membrane protein 140, GMP-140, GMP140, CD62P, CD62 antigen-like family member P, CD antigen CD62P, CD62, granulocyte membrane protein, selectin P (granule membrane protein 140kDa, antigen CD62)

Produktbeschreibung

Rabbit anti-P-Selectin/CD62P Recombinant Monoclonal Antibody

Produkteigenschaften

• Klonalität: Recombinant
• Konzentration: 1000 µg/ml
• Klon-Bezeichnung: [BLR267L]
• UniProt: P16109
• Puffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Reinheit: Recombinant antibody was purified from cell culture supernatant
• Formulierung: Whole IgG
• Target-Kategorie: P-Selectin/CD62P

Anwendungsbeschreibung

• Application Verdünnung: Immunocytochemistry (ICC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. Immunohistochemistry (IHC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Anwendungsbeschreibung: Application Notes: All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight

Bilder

Detection of human P-Selectin/CD62P by immunocytochemistry. Sample: FFPE section of HEL 92.1.7 cells. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784576). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human P-Selectin/CD62P by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784576). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human P-Selectin/CD62P (red) by immunofluorescence. Sample: FFPE section of metastatic lymph node. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784576) used at 1:100. Secondary: Invitrogen Goat anti-rabbit IgG Alexa Fluor(TM) Plus 647 1:400. Counterstain: DAPI (blue).

Detection of human P-Selectin/CD62P by western blot. Samples: Whole cell lysate (25 µg) from HEK293T, Platelets, Jurkat, HEL 92.1.7, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-P-Selectin/CD62P recombinant monoclonal antibody (orb1784576) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.