Rabbit anti-E-Selectin/CD62E Recombinant Monoclonal Antibody, Clone: [BLR295L], Unconjugated

Produktübersicht

• Artikelname: Rabbit anti-E-Selectin/CD62E Recombinant Monoclonal Antibody, Clone: [BLR295L], Unconjugated
• Artikelnummer: BYT-ORB1806428
• Hersteller Artikelnummer: orb1806428
• Alternativnummer: BYT-ORB1806428-100
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FC, ICC, IHC, IP, WB
• Spezies Reaktivität: Human
• Immunogen: Residues 22-555 (ECD)
• Konjugation: Unconjugated
• Alternative Synonym: endothelial adhesion molecule 1, endothelial leukocyte adhesion molecule 1, Leukocyte-endothelial cell adhesion molecule 2, leukocyte endothelial cell adhesion molecule 2, LECAM2, ESEL, CD antigen CD62E, ELAM1, ELAM, CD62E, CD62 antigen-like family member E, E-selectin, ELAM-1

Produktbeschreibung

Rabbit anti-E-Selectin/CD62E Recombinant Monoclonal Antibody

Produkteigenschaften

• Klonalität: Recombinant
• Konzentration: 1000 µg/ml
• Klon-Bezeichnung: [BLR295L]
• UniProt: P16581
• Puffer: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Formulierung: Whole IgG
• Target-Kategorie: E-Selectin/CD62E

Anwendungsbeschreibung

• Application Verdünnung: WB: 1:1000, IP: 6 µl/mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Anwendungsbeschreibung: Application Notes: Format: Whole IgG

Bilder

Detection of human E-Selectin/CD62E (shaded) in TNF-alpha treated HUVEC cells by flow cytometry. Antibody: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (orb1806428) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG.

Detection of human E-Selectin/CD62E by immunocytochemistry. Sample: FFPE section of HUVEC-C cells treated with TNF-alpha (left) and mock treated HUVEC-C cells (right). Antibody: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (orb1806428). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human E-Selectin/CD62E by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (orb1806428). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human E-Selectin/CD62E by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction, 20% of IP loaded) from HUVEC-C cells treated with 10ng/mL TNFalpha. Antibodies: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (orb1806428) used for IP at 6 µl/mg lysate.

Detection of human E-Selectin/CD62E by western blot. Samples: Whole cell lysate (50 µg) from HUVEC-C cells untreated (-) or treated (+) with 10ng/mL TNFalpha. Antibody: Rabbit anti-E-Selectin/CD62E recombinant monoclonal antibody (orb1806428) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.