SARS-CoV-2 spike protein (RBD) neutralizing (C-A11) rabbit mAb Antibody, Clone: [CoV2-CA11], Unconjugated, Rabbit, Monoclonal

Produktübersicht

• Artikelname: SARS-CoV-2 spike protein (RBD) neutralizing (C-A11) rabbit mAb Antibody, Clone: [CoV2-CA11], Unconjugated, Rabbit, Monoclonal
• Artikelnummer: BYT-ORB1945971
• Hersteller Artikelnummer: orb1945971
• Alternativnummer: BYT-ORB1945971-20,BYT-ORB1945971-200
• Hersteller: Biorbyt
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: FA
• Spezies Reaktivität: Other
• Immunogen: recombinant protein (RBD)
• Konjugation: Unconjugated
• Alternative Synonym: Binding to ACE-2 receptor, RBD domain, SARS-CoV-2 variants, South Africa variant B.1.351, Spike protein S1, Spike protein S2, U.K variant B.1.1.7, sars-cov-2

Produktbeschreibung

SARS-CoV-2 spike protein (RBD) neutralizing (C-A11) rabbit mAb

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 0.5 mg/mL
• Klon-Bezeichnung: [CoV2-CA11]
• UniProt: P0DTC2
• Puffer: 1X PBS
• Formulierung: Liquid

Anwendungsbeschreibung

• Application Verdünnung: This antibody recognizes the SARS-CoV-2 Spike Protein RBD domain and inhibits the interaction between SARS-CoV-2 RBD and ACE2. Can be paired with other antibody for detection in sandwich ELISA format.

Bilder

Flow cytometry neutralizing assay: C-A11 displayed dose dependent blocking of spike proteins (wild type and UK variant) to ACE2 expressing cells.

Affinity assessment of SARS-CoV-2 C-A11 IgG to wild type and B.1.1.7 (UK) S1 + S2 trimers. C-A11 possesses very low (pM) KD affinity to both variants.

Flow cytomery neutralizing assay of C-A11 (plotted graphs): ACE2 transfected cells were incubated with biotinylated spike protein (0.5 ug/mL) in the presence or absence of antibody. Binding of spike protein was detected with DyLight488 conjugated StreptAv

Binding of C-A11 to SARS-CoV-2 variants (UK and South Africa): C-A11 shows good cross reactivity to both variants B.1.1.7 (UK) and B.1.351 (S. Africa).