Sirius Red/Fast Green Collagen Staining Kit
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|Artikelname:||Sirius Red/Fast Green Collagen Staining Kit|
|Sirius Red/Fast Green Collagen Staining Kit|
Sirius Red and Fast Green is a dye combination used to distinguish collagen from its surrounding materials.
Sirius Red specifically binds the [Gly-X-Y]n helical structure of fibrillar collagens, regardless of collagen type or species, whereas Fast Green binds to non-collagenous proteins. By exploiting the unique features of these two dyes, Chondrex, Inc. provides a simple semi-quantitative assay kit to determine the amounts of collagen and non-collagenous proteins in cultured cell layers and tissue sections.
Because this assay does not require collagen solubilization, it can be used to measure total collagen content in various tissues. As Sirius Red and Fast Green have absorptions at 540 nm and 605 nm respectively, the OD values of the extracted dyes can be used to calculate the collagen and non-collagenous protein content of samples. For general histological studies in which tissue sections are 10-20 μm thick, the assay sensitivity for collagen and non-collagenous proteins is greater than 3 μg/section and 50 μg/section, respectively. This kit contains enough reagents to stain 30-50 samples.
|Detektionsbereich:||Collagen: 3 µg/section Other Proteins: 50 µg/section|