DDX3X Antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: DDX3X Antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: CSB-PA006621LA01HU
• Hersteller Artikelnummer: CSB-PA006621LA01HU
• Alternativnummer: CSB-PA006621LA01HU-100UG, CSB-PA006621LA01HU-50UG
• Hersteller: Cusabio
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, IP, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Konjugation: Unconjugated
• Alternative Synonym: ATP dependent RNA helicase DDX3X antibody, ATP-dependent RNA helicase DDX3X antibody, CAP Rf antibody, DBX antibody, DDX14 antibody, DDX3X antibody, DDX3X_HUMAN antibody, DEAD (Asp Glu Ala Asp) box polypeptide 3 X linked antibody, DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked antibody, DEAD box antibody, DEAD box protein 3 antibody, DEAD box protein 3 X-chromosomal antibody, DEAD box X isoform antibody, DEAD box, X isoform antibody, DEAD/H (Asp Glu Ala Asp/His) box polypeptide 3 antibody, DEAD/H box 3 antibody, DEAD/H box 3, X-linked antibody, Helicase like protein 2 antibody, Helicase-like protein 2 antibody, HLP2 antibody, X isoform antibody, X-chromosomal antibody

Produkteigenschaften

• Klonalität: Polyclonal
• UniProt: O00571
• Puffer: Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Reinheit: >95%, Protein G purified
• Formulierung: Liquid
• Target-Kategorie: DDX3X

Anwendungsbeschreibung

• Application Verdünnung: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000

Bilder

Immunofluorescence staining of Hela cell with CSB-PA006621LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of HepG2 cell with CSB-PA006621LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Western Blot
Positive WB detected in:293 whole cell lysate, HepG2 whole cell lysate, JK whole cell lysate, MCF7 whole cell lysate, Mouse Brain tissue lysate, Rat Brain tissue lysate
All lanes:DDX3 Antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 74 kDa
Observed band size: 74 kDa

Immunohistochemistry analysis of human adrenal gland tissue using CSB-PA006621LA01HU at dilution of 1:100

Immunoprecipitating DDX3X in Jurkat whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA006621LA01HU in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA006621LA01HU (8µg) + Jurkat whole cell lysate (500µg)
Lane 3: Jurkat whole cell lysate (10µg)