Signal-Seeker(TM) Ubiquitin Detection Kit (30 assays)

Artikelnummer: CYS-BK161
Artikelname: Signal-Seeker(TM) Ubiquitin Detection Kit (30 assays)
Artikelnummer: CYS-BK161
Hersteller Artikelnummer: BK161
Alternativnummer: CYS-BK161-KIT,CYS-BK161-30
Hersteller: Cytoskeleton
Kategorie: Kits/Assays
Signal-Seeker(TM) Ubiquitin Detection Kit (30 assays)
Swiss 3T3 cells were pre-treated with MG-132, then either untreated or treated with CNF1 (CN04) for 3 hours prior to lysis with BlastR buffer. The BK161 kit was utilized to perform the IP on 300 μg of lysate per condition. Lane 1: 3 μg input untreated lysate, Lane 2: Ubiquitin Affinity beads (UBA01) plus untreated lysate, Lane 3: UBA01 plus CN04 treated lysate, Lane 4: ubiquitin control beads (CUB02) plus untreated lysate, Lane 5: CUB02 plus CN04 treated lysate. Samples were analyzed for Rac1 ubiquitination using an anti-Rac1 antibody.