Anti-IDH1 (Hu) from Rat (W09)

Produktübersicht

• Artikelname: Anti-IDH1 (Hu) from Rat (W09)
• Artikelnummer: DNA-DIA-W09
• Hersteller Artikelnummer: DNA-DIA-W09
• Alternativnummer: DNA-DIA-W09
• Hersteller: dianova
• Wirt: Rat
• Kategorie: Antikörper
• Applikation: IHC
• Spezies Reaktivität: Human
• Immunogen: Peptidsequenz 4-120 von humanem IDH1
• Konjugation: Unconjugated
• Alternative Synonym: IDH1,Isocitrate dehydrogenase [NADP] (cytoplasmic),PICD,IDP,Oxalosuccinate decarboxylase,NADP(+)-specific ICDH,Cytosolic NADP-isocitrate dehydrogenase,Isocitrate Dehydrogenase 1, Soluble

Produktbeschreibung

Anti-IDH1 (Hu) aus Ratte (W09), IgG2a, Unconjugated, Monoclonal

Reactivity

Isocitrate dehydrogenase (IDH) is an important enzyme in the citric acid cycle and catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate and CO2 while converting NAD+ to NADH. Heterozygous point mutations of IDH1 codon 132 are frequent in World Health Organization (WHO) grade II and III gliomas. IDH1 R132H mutations occur in approximately 70% of astrocytomas and oligodendroglial tumors. Mouse antibody clone H09 (product number #DIA-H09) reacts specifically with the isocitrate dehydrogenase 1 (IDH1) R132H point mutation in tissue sections from formalin-fixed brain tumor specimens. Therefore this antibody is highly useful for tumor classification. Rat antibody clone W09 detects both wild type (wt) and point mutated IDH1 in glioblastoma (anti-pan-IDH1) and serves as control for the IDH1 point mutation specific mouse antibody clone H09. Moreover, Tan et al. (Mol Cell Proteomics, 2011) have identified IDH1 as a potential diagnostic and prognostic biomarker for Non-small-cell Lung Cancer (NSCLC). These findings suggest that anti-IDH1wt antibody could be used as a histochemical biomarker for prognosis prediction of NSCLC.

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: (0,2mg/ml)
• Klon-Bezeichnung: W09
• Isotyp: IgG2a
• NCBI: 3417
• UniProt: O75874
• Puffer: in PBS, 2% BSA, 0,05%NaN3
• Target-Kategorie: IDH1

Anwendungsbeschreibung

• Application Verdünnung: Histo-/Zytochemie 1:20 - 1:100,Western Blot (WB): 1:500

Bilder

No-reaction-of-IDH1-R132H-mutation-specific-antibody-clone-H09_200x

No-reaction-of-IDH1-R132H-mutation-specific-antibody-clone-H09_400x

strong-reaction-of-anti-pan-IDH1_200x

strong-reaction-of-anti-pan-IDH1_400x