The rapid photobleaching of cell probes labelled with fluorochromes has always hindered the qualitative and quantitative assessment of the label. ImmunoSelect® Antifading Mounting Medium is developed for fluorescence-microscopical use combining strong initial fluorescence with drastical reduction of photobleaching over time. In contrast to several media ImmunoSelect® Antifading Mounting Medium does not exhibit autofluorescence at any common wavelength. ImmunoSelect® Antifading Mounting Medium is an aqueous mountant which does not solidify directly, but remains a viscous liquid for several days on the slide. To mount cellular material on the slide one drop of medium is sufficient. After a cover slip is applied, the medium disperses over the entire section. When mounted slides are stored at 4°C in the dark, fluorescence will remain for many weeks.

ImmunoSelect® Antifading Mounting Medium is ready to use and available with or without DNA Fluorescent Dyes (DAPI or PI (propidium iodide)).

 

Immunoselect Antifading Mounting Medium is tested for several fluorescence dyes:
• Fluorescein derivatives, eg. FITC
• Rhodamine derivatives, eg. Rhodamin Red-X, TRITC, Texas Red
• All common fluorescent dyes, eg. Alexa Fluor dyes, DyLight dyes, Cyanine dyes
• Phycobilliproteins, eg. PE
• DAPI
• Hoechst 33358 and 3334
• Propidium Iodide