Anti-Chicken IgG Antibody generated in donkey detects chicken IgY. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fung
Klonalität:
Polyclonal
Konzentration:
1.2
Isotyp:
Ig
Puffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reinheit:
Anti-Chicken IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Chicken IgG and Chicken Serum. No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins.
Formulierung:
Liquid (sterile filtered)
Formel:
20 mM K3PO4,150 mM NaCl,pH 7,2,sterile filtered,Azide/BSA free
Target-Kategorie:
Chicken
Antibody Type:
Secondary Antibody
Application Verdünnung:
ELISA Dilution: 1:20,000 - 1:100,000, Immunohistochemistry Dilution: 1:1,000 - 1:5,000, Western Blot Dilution: 1:2,000 - 1:10,000
Anwendungsbeschreibung:
Anti-Chicken IgG (H&L) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
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