GAPDH antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: GAPDH antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: GTX100118
• Hersteller Artikelnummer: GTX100118
• Alternativnummer: GTX100118-100,GTX100118-25
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ICC, IHC-P, IP, WB
• Spezies Reaktivität: Bacteria, Bovine, Canine, Drosophila, E. coli, Fish, Gallus, Hamster, Human, Insect, Mammal, Monkey, Mouse, Other, Plant, Porcine, Rabbit, Rat, Yeast, Zebrafish
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GAPDH. The exact sequence is proprietary.
• Konjugation: Unconjugated
• Alternative Synonym: glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP

Produktbeschreibung

GAPDH antibody recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein, a glycolytic enzyme of ~37 kDa that forms a tetramer. Because of its widespread expression, GAPDH is commonly used as an internal control for various protein assays, part

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.11 mg/ml (Please refer to the vial label for the specific concentration.)
• Molekulargewicht: 36
• NCBI: 2597
• UniProt: P04406
• Puffer: 1XPBS, 1% BSA, 20% Glycerol (pH7), 0.025% ProClin 300.
• Reinheit: Purified by antigen-affinity chromatography.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:5000-1:100000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX100118 WB Image

GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of GAPDH protein from 293T whole cell extracts using 5 μg of GAPDH antibody (GTX100118).
Western blot analysis was performed using GAPDH antibody (GTX100118).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX100118) diluted at 1:5000.

GAPDH antibody detects GAPDH protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
10% SDS-PAGE
GAPDH antibody (GTX100118) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human duodenum.
GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GAPDH antibody detects GAPDH protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GAPDH antibody detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
GAPDH stained by GAPDH antibody (GTX100118) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min