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GTX101277 ICC/IF Image |
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HMGB1 antibody detects HMGB1 protein at nucleus on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. HMGB1 antibody (GTX101277) dilution: 1:1000.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min |
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HMGB1 antibody detects HMGB1 protein at nucleus on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. HMGB1 antibody (GTX101277) dilution: 1:1000.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min |
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Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX101277) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
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HMGB1 antibody detects HMGB1 protein at nucleus in mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. HMGB1 antibody (GTX101277) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
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Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX101277) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
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HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis. Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGB1 protein stained by HMGB1 antibody (GTX101277) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm. |
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HMGB1 antibody detects HMGB1 protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. HMGB1 antibody (GTX101277) dilution: 1:1000. _x000D_ Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min |
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HMGB1 antibody detects HMGB1 protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract 12% SDS-PAGE HMGB1 antibody (GTX101277) dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
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HMGB1 antibody detects HMGB1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGB1 protein stained by HMGB1 antibody (GTX101277) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.
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HMGB1 antibody detects HMGB1 protein by western blot analysis. A. 30 μg NIH-3T3 whole cell lysate/extract B. 30 μg JC whole cell lysate/extract C. 30 μg BCL-1 whole cell lysate/extract D. 30 μg C2C12 whole cell lysate/extract E. 30 μg Raw264.7 whole cell lysate/extract 12% SDS-PAGE HMGB1 antibody (GTX101277) dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
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Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody (GTX101277) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |