Galectin 3 antibody, Unconjugated, Rabbit, Polyclonal

Artikelnummer: GTX113486
Artikelname: Galectin 3 antibody, Unconjugated, Rabbit, Polyclonal
Artikelnummer: GTX113486
Hersteller Artikelnummer: GTX113486
Alternativnummer: GTX113486-100,GTX113486-25
Hersteller: GeneTex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, ICC, IHC-P, WB
Spezies Reaktivität: Canine, Human, Rat
Immunogen: Recombinant protein encompassing a sequence within the center region of human Galectin 3. The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: galectin 3 , CBP35 , GAL3 , GALBP , GALIG , L31 , LGALS2 , MAC2
Klonalität: Polyclonal
Konzentration: 0.85 mg/ml (Please refer to the vial label for the specific concentration.)
Molekulargewicht: 26
NCBI: 3958
UniProt: P17931
Puffer: 1XPBS (pH7), 20% Glycerol, 0.025% ProClin 300.
Reinheit: Purified by antigen-affinity chromatography.
Formulierung: Liquid
Anwendungsbeschreibung: WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Sandwich ELISA: . *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX113486 ELISA Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody (GTX113486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (30 ug of whole cell lysate)
A: Non-transfected 293T lysates
B: LGALS3 transfected 293T lysates
12% SDS PAGE
GTX113486 diluted at 1:10000
Wild-type (WT) and Galectin 3 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody (GTX113486) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Galectin3 antibody detects Galectin3 protein at cytoplasm by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: Galectin3 protein stained by Galectin3 antibody (GTX113486) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using Galectin3(GTX113486) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Galectin3 antibody detects Galectin3 protein by western blot analysis.
A. 50 μg rat colon lysate/extract
12 % SDS-PAGE
Galectin3 antibody (GTX113486) dilution: 1:10000
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody (GTX113486) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.