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GTX135356 WB Image |
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Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) spike antibody (GTX135356) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
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Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) spike antibody (GTX135356) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody. |
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Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) spike protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) spike antibody (GTX135356) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody. |
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SARS-CoV-2 (COVID-19) Spike antibody detects SARS-CoV-2 (COVID-19) Spike protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SARS-CoV-2 (COVID-19) Spike stained by SARS-CoV-2 (COVID-19) Spike antibody (GTX135356) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920). |
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Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 spike (full length) transfected (GTX535664) 293T whole cell extracts using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX135356 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody. |
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SARS-CoV-2 (COVID-19) spike antibody detects SARS-CoV-2 (COVID-19) spike protein by immunohistochemical analysis.Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) Spike FFPE Cell Pellet Block.Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV-2 (COVID-19) spike antibody (GTX135356) diluted at 1:1000.Red: SARS-CoV / SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
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Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) spike antibody (GTX135356) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |