SARS-CoV-2 (COVID-19) Spike S2 / S2 antibody, Unconjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: SARS-CoV-2 (COVID-19) Spike S2 / S2 antibody, Unconjugated, Rabbit, Polyclonal
• Artikelnummer: GTX135386
• Hersteller Artikelnummer: GTX135386
• Alternativnummer: GTX135386-100,GTX135386-25
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, ICC, WB
• Spezies Reaktivität: Virus
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of SARS-CoV-2 Spike (S2). (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.
• Konjugation: Unconjugated
• Alternative Synonym: 2019-nCoV Spike, COVID-19 Spike, SARS-CoV-2 Spike, covid-19, sars-cov-2

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Sensitivitaet: This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.
• Puffer: 1XPBS (pH7), 20% Glycerol, 0.025% ProClin 300.
• Reinheit: Purified by antigen-affinity chromatography.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX135386 WB Image

SARS-CoV-2?(COVID-19) Spike S2 (ECD) protein, mouse IgG Fc tag (1 μg, GTX135684-pro) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 / S2' antibody (GTX135386) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike antibody detects SARS-CoV-2 (COVID-19) Spike protein by immunofluorescent analysis.
Sample: Mock and SARS-CoV-2 Spike-transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike stained by SARS-CoV-2 (COVID-19) Spike antibody (GTX135386) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) spike S2 ECD protein using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX135386 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant SARS-CoV-2 (COVID-19) spike S2 ECD protein at concentrations ranging from 0.0625 μg/mL to 2 μg/mL. The coated protein is detected with (GTX135386) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S2 / S2' antibody (GTX135386) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.