SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.617.2 / Delta variant, His tag (active), Unconjugated

Produktübersicht

• Artikelname: SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.617.2 / Delta variant, His tag (active), Unconjugated
• Artikelnummer: GTX136332-PRO
• Hersteller Artikelnummer: GTX136332-pro
• Alternativnummer: GTX136332-PRO-100
• Hersteller: GeneTex
• Kategorie: Proteine/Peptide
• Applikation: ELISA, Receptor Binding Assays, WB
• Spezies Reaktivität: Virus
• Konjugation: Unconjugated
• Alternative Synonym: B.1.617.2 Spike, COVID-19 Delta variant Spike, Delta variant Spike, SARS-CoV-2 Delta variant Spike, covid-19, sars-cov-2

Produktbeschreibung

SARS-CoV-2 (COVID-19) Spike RBD Protein, B.1.617.2 / Delta variant, His tag (active), Unconjugated

Produkteigenschaften

• Konzentration: 0.58 mg/ml (Please refer to the vial label for the specific concentration.)
• Puffer: PBS, no preservative.
• Expression System: HEK293 cells
• Formulierung: Liquid
• Sequenz: SARS-CoV-2 Spike RBD of QHD43416.1 (319-541 a.a) with L452R and T478K mutations and His tag at the C-terminus

Bilder

GTX136332-pro ELISA Image

Functional ELISA analysis of immobilized recombinant Spike RBD proteins of SARS-CoV-2 (COVID-19) derived from different strains (Delta variant B.1.617.2 or Wide type strain) (2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (0.16-117.19 nM). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Inhibition analysis of immobilized recombinant RBD proteins derived from different strains of SARS-CoV-2 virus (Wild type or B1.617.2 delta variant) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL) ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) (0.09-66.67 nM). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

1 μg of GTX136332-pro SARS-CoV-2 (COVID-19) Spike RBD (L452R, T478K Mutant) protein, His tag protein was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

SARS-CoV-2 (COVID-19) Spike RBD (L452R, T478K Mutant) protein, His tag protein (GTX136332-pro, 0.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000.