Dengue virus NS5 protein antibody [GT361], IgG1, Unconjugated, Mouse, Monoclonal

Produktübersicht

• Artikelname: Dengue virus NS5 protein antibody [GT361], IgG1, Unconjugated, Mouse, Monoclonal
• Artikelnummer: GTX629447
• Hersteller Artikelnummer: GTX629447
• Alternativnummer: GTX629447-100,GTX629447-25
• Hersteller: GeneTex
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: ICC, WB
• Spezies Reaktivität: Virus
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Dengue virus Type 2 NS5 protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.
• Konjugation: Unconjugated
• Alternative Synonym: Dengue Virus Non-structural protein 5 , Dengue Virus NS5 , DENV Non-structural protein 5 , DENV NS5

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Klon-Bezeichnung: [GT361]
• Molekulargewicht: 103
• Isotyp: IgG1
• Sensitivitaet: This antibody does not cross-react with JEV Envelope protein.
• Puffer: 1XPBS (pH7), 20% Glycerol, no Preservative.
• Reinheit: Affinity purified by Protein G.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: WB: 1:1000-1:10000. ICC/IF: 1:100-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX629447 ICC/IF Image

NS5 (Dengue virus 2) antibody [GT361] detects NS5 (Dengue virus 2) protein at nucleus by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in paraformaldehyde.
Green: NS5 (Dengue virus 2) protein stained by NS5 (Dengue virus 2) antibody [GT361] (GTX629447) diluted at 1:2000.
Blue: Hoechst 33342 staining.

NS5 (Dengue virus 2) antibody [GT361] detects NS5 (Dengue virus 2) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extract
B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
7.5% SDS-PAGE
NS5 (Dengue virus 2) antibody [GT361] (GTX629447) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

NS5 (Dengue Virus 2) antibody [GT361] detects NS5 protein by western blot analysis.
A. 30 μg BHK21 whole cell lysate/extract
B. 30 μg whole cell lysate/extract of Dengue virus 2 infected BHK21 cells
C. 30 μg whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells
10% SDS-PAGE
NS5 (Dengue Virus 2) antibody [GT361] (GTX629447) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus Type 2 NS5 protein antibody [GT361] (GTX629447) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.