SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263], IgG, Clone: Monoclonal, Unconjugated, Rabbit

Artikelnummer: GTX635672
Artikelname: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263], IgG, Clone: Monoclonal, Unconjugated, Rabbit
Artikelnummer: GTX635672
Hersteller Artikelnummer: GTX635672
Alternativnummer: GTX635672-25,GTX635672-100
Hersteller: GeneTex
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ICC, IHC-P, ELISA, WB
Spezies Reaktivität: Virus
Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of SARS-CoV-2 (COVID-19) spike (S1) (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.
Konjugation: Unconjugated
Alternative Synonym: COVID-19, SARS-CoV-2 Spike, SARS-COV, COVID, NCOV, 2019-nCoV Spike, COVID19, COV2, CORONA, COVID-19 Spike, SARS-COV-2, COV-2
Klonalität: Monoclonal
Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Klon-Bezeichnung: Monoclonal
Isotyp: IgG
Puffer: PBS
Reinheit: Affinity purified by protein A.
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 spike (full length) transfected (GTX535664) 293T whole cell extracts using SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] detects SARS-CoV-2 (COVID-19) Spike protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein, and SARS-CoV spike S1 subunit protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein, and SARS-CoV spike S1 subunit protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) (2.4*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.365 nM
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.