SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344], unconjugated, rabbit, Monoclonal

Artikelnummer: GTX635679
Artikelname: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344], unconjugated, rabbit, Monoclonal
Artikelnummer: GTX635679
Hersteller Artikelnummer: GTX635679
Alternativnummer: GTX635679-100,GTX635679-25
Hersteller: GeneTex
Wirt: rabbit
Kategorie: Antikörper
Applikation: Ihc-Fr, WB, ICC, ELISA, Ihc-P, Ihc-P, ELISA
Spezies Reaktivität: Virus
Immunogen: Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein. (SARS-CoV-2 (strain Wuhan-Hu-1))
Konjugation: unconjugated
Alternative Synonym: COVID19, COV-2, COVID-19, 2019-nCoV Nucleocapsid | SARS-CoV-2 Nucleocapsid | COVID-19 Nucleocapsid | 2019-nCoV NP | SARS-CoV-2 NP | COVID-19 NP, COVID, CORONA, SARS-COV, NCOV, COV2, SARS-COV-2
SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344], IgG, Unconjugated, Rabbit, Monoclonal
Klonalität: Monoclonal
Konzentration: 1mg/ml
Klon-Bezeichnung: HL344
Isotyp: IgG
Pubmed: 32876341, 34425707,
Puffer: PBS
Quelle: SARS Coronavirus 2
Reinheit: Affinity purified by protein A.
Antibody Type: Recombinant Antibody
Anwendungsbeschreibung: WB (1:5000-1:20000), ICC/IF (1:100-1:1000), IHC-P (1:500-1:1000),
SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) as detection antibody at concentration of 1 μg/mL.
Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635679. Please contact us for custom HRP-conjugated antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (2.4*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.074 nM
Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) and (GTX135592-pro) at concentrations ranging from 0.015 μg/mL to 1 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.
SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis of SARS-CoV-2 infected human placenta (left) and normal human placenta (right).

The IHC-P was performed by HISTOWIZ.
SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis of SARS-CoV-2 infected human lung.

The IHC-P was performed by HISTOWIZ.