Influenza B virus Nucleoprotein antibody [HL1073], Unconjugated, Rabbit, Monoclonal

Produktübersicht

• Artikelname: Influenza B virus Nucleoprotein antibody [HL1073], Unconjugated, Rabbit, Monoclonal
• Artikelnummer: GTX636194
• Hersteller Artikelnummer: GTX636194
• Alternativnummer: GTX636194-100,GTX636194-25
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, ICC, LF, WB
• Spezies Reaktivität: Virus
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of Influenza B virus Nucleoprotein (B/Taiwan/753/2005). The exact sequence is proprietary.
• Konjugation: Unconjugated

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Klon-Bezeichnung: [HL1073]
• Puffer: PBS, no preservative.
• Reinheit: Affinity purified by Protein A.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX636194 Lateral Flow Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Influenza B virus Nucleoprotein antibody (GTX636100) detects overexpressed Influenza virus Nucleoprotein protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Influenza B virus Nucleoprotein stained by Influenza B virus Nucleoprotein antibody (GTX636100) diluted at 1:2000.

Immunofluorescent analysis of influenza virus infected cells using Influenza B virus Nucleoprotein antibody (GTX636194).
Sample: Influenza A and B Virus infected cells slide.
Green: Influenza B virus Nucleoprotein antibody (GTX636194) diluted at 1:100.

Indirect ELISA analysis was performed by coating a plate with viral lysates (1 μg) derived from different strains of influenza B virus or influenza A virus and probing with the specified influenza B virus nucleoprotein antibodies (1 μg/ml). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Indirect ELISA analysis was performed by coating a plate with recombinant influenza B virus nucleoprotein (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro) (50 ng), and probing with the specified influenza B virus nucleoprotein antibodies at the indicated concentrations. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Indirect ELISA analysis was performed by coating a plate with viral lysates (1 μg) derived from different strains of influenza B virus or influenza A virus and probing with the specified influenza B virus nucleoprotein antibodies (1 μg/ml). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Influenza B viral lysates (1 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below._x000D__x000D_
Capture: Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (5 μg/mL) _x000D_
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (1 μg/mL)._x000D_

Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.

Indirect ELISA analysis performed by coating plate with recombinant full-length Influenza B virus NP (nucleoprotein) protein, DDDDK tag (GTX135867-pro) (50 ng). Coated protein was probed with Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (20000-4.77*10^-3 pM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
EC50 : 29.94 pM

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below._x000D__x000D_
Capture: Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (5 μg/mL) _x000D_
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) (1 μg/mL)._x000D_

Please notice that GTX636099 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using antibodies as below._x000D__x000D_
Capture: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (5 μg/mL) _x000D_
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (1 μg/mL)_x000D_

Please notice that GTX636194 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636100. Please contact us for custom HRP-conjugated antibody.

Indirect ELISA analysis performed by coating plate with viral lysate derived from different strains of Influenza A virus (i.e., A/California/07/09 (H1N1)) and Influenza B virus (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Malaysia/2506/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10; B/Lee/40) (1-0.2 μg). Coated protein was probed with Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Sandwich ELISA detection of recombinant Influenza B virus Nucleoprotein (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro) using antibodies as below._x000D__x000D_
Capture: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (5 μg/mL) _x000D_
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (1 μg/mL)_x000D_

Please notice that GTX636194 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636100. Please contact us for custom HRP-conjugated antibody.

Sandwich ELISA detection of viral lysate derived from different strains of Influenza A virus (i.e., A/California/07/09 (H1N1)) and Influenza B virus (i.e., B/Brisbane/33/08; B/Florida/02/06; B/Florida/04/06; B/Florida/07/04; B/Massachusetts/2/12; B/Panama/45/90; B/Wisconsin/1/10) using antibodies as below._x000D__x000D_
Capture: Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) (5 μg/mL) _x000D_
Detection: HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) (1 μg/mL)_x000D_

Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) as capture antibody at concentration of 5 μg/mL and HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX636194 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636099. Please contact us for custom HRP-conjugated antibody.

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) as capture antibody at concentration of 5 μg/mL and HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX636099 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194) as capture antibody at concentration of 5 μg/mL and HRP-conjugated Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX636100 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636194. Please contact us for custom HRP-conjugated antibody.