Influenza A virus Nucleoprotein antibody [HL1089], Unconjugated, Rabbit, Monoclonal

Produktübersicht

• Artikelname: Influenza A virus Nucleoprotein antibody [HL1089], Unconjugated, Rabbit, Monoclonal
• Artikelnummer: GTX636247
• Hersteller Artikelnummer: GTX636247
• Alternativnummer: GTX636247-100,GTX636247-25
• Hersteller: GeneTex
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, ICC, IHC-P, LF, WB
• Spezies Reaktivität: Virus
• Immunogen: Recombinant protein encompassing a sequence within the center region of Influenza A virus Nucleoprotein (A/Kansas/14/2017(H3N2)). The exact sequence is proprietary.
• Konjugation: Unconjugated

Produkteigenschaften

• Klonalität: Monoclonal
• Konzentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Klon-Bezeichnung: [HL1089]
• Sensitivitaet: This antibody is specific for Influenza A virus Nucleoprotein protein (H1N1, H3N2, H5N8, and H10N3), and it does not cross-react with Influenza B virus Nucleoprotein protein.
• Puffer: PBS, no preservative.
• Reinheit: Affinity purified by Protein A.
• Formulierung: Liquid

Anwendungsbeschreibung

• Anwendungsbeschreibung: *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Bilder

GTX636247 Lateral Flow Image

Indirect ELISA analysis was performed by coating a plate with recombinant influenza A virus nucleoprotein (A/Kansas/2017/H3N2), DDDDK Tag (GTX135903-pro) (50 ng), and probing with the specified influenza A virus nucleoprotein antibodies at the indicated concentrations. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Indirect ELISA analysis was performed by coating a plate with viral lysates (1 μg) derived from different strains of influenza A virus or influenza B virus and probing with the specified influenza A virus nucleoprotein antibodies (1 μg/ml). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Immunofluorescent analysis of influenza virus infected cells using Influenza A virus Nucleoprotein antibody (GTX636247).
Sample: Influenza A and B Virus infected cells slide.
Green: Influenza A virus Nucleoprotein antibody (GTX636247) diluted at 1:100.

Influenza A virus Nucleoprotein antibody (GTX636247) detects overexpressed Influenza virus Nucleoprotein protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Influenza A virus Nucleoprotein stained by Influenza A virus Nucleoprotein antibody (GTX636247) diluted at 1:2000.

Detection of recombinant influenza A virus nucleoprotein (NP) (A/WSN/1933 (H1N1)), DDDDK Tag (GTX135868-pro) by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs.

HL1078 : Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199)
HL1089 : Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247)
HL1103 : Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318)
Sample : Influenza A virus Nucleoprotein (A/WSN/1933 (H1N1)), DDDDK Tag (GTX135868-pro)

Indirect ELISA analysis performed by coating plate with recombinant full-length Influenza A virus NP (nucleoprotein) antibody (GTX135903-pro) (50 ng). Coated protein was probed with Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) (20000-4.77*10-3 pM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

EC50 : 37.93 pM

Sandwich ELISA detection of viral lysate derived from different strains of Influenza A virus (i.e., A/Brisbane/10/07 (H3N2); A/Perth/16/09 (H3N2); A/Texas/50/12 (H3N2); A/Wisconsin/67/05 (H3N2); A/Hong Kong/8/68 (H3N2)) and Influenza B virus (i.e., B/Florida/02/06) using antibodies as below._x000D__x000D_
Capture: Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318) (5 μg/mL) _x000D_
Detection: HRP-conjugated Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) (1 μg/mL)_x000D_

Please notice that GTX636247 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636318. Please contact us for custom HRP-conjugated antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Indirect ELISA analysis performed by coating plate with viral lysate derived from different strains of Influenza A virus (i.e., A/Brisbane/59/07 (H1N1); A/New Cal/20/99 (H1N1); A/Puerto Rico/8/34 (H1N1); A/Taiwan/42/06 (H1N1); A/California/07/09 (H1N1); A/Mexico/4108/09 (H1N1); A/New York/01/09 (H1N1); A/New York/02/09 (H1N1); A/New York/03/09 (H1N1); A/Canada/6294/09 (H1N1); A/Singapore/63/04 (H1N1); A/Brisbane/10/07 (H3N2); A/Perth/16/09 (H3N2); A/Texas/50/12 (H3N2); A/Wisconsin/67/05 (H3N2); A/Hong Kong/8/68 (H3N2)) and Influenza B virus (i.e., B/Florida/02/06) (1-0.2 μg). Coated protein was probed with Influenza A virus NP (nucleoprotein) antibody [HL1089] (GTX636247) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Sandwich ELISA detection of viral lysate derived from different strains of Influenza A virus (i.e., A/Brisbane/59/07 (H1N1); A/New Cal/20/99 (H1N1); A/Puerto Rico/8/34 (H1N1); A/Taiwan/42/06 (H1N1); A/Singapore/63/04 (H1N1); A/California/07/09 (H1N1); A/Mexico/4108/09 (H1N1); A/New York/01/09 (H1N1); A/New York/02/09 (H1N1); A/New York/03/09 (H1N1); A/Canada/6294/09 (H1N1)) and Influenza B virus (i.e., B/Florida/02/06) using antibodies as below._x000D__x000D_
Capture: Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318) (5 μg/mL) _x000D_
Detection: HRP-conjugated Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) (1 μg/mL)_x000D_

Please notice that GTX636247 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636318. Please contact us for custom HRP-conjugated antibody.

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza A virus (i.e., A/Kansas/2017 (H3N2); A/California/2009 (H1N1); A/Hong Kong/2671/2019 (H3N2)) and Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987)) using antibodies as below._x000D__x000D_
Capture : Influenza A virus NP (nucleoprotein) antibody [HL1103] (GTX636318) (5 μg/mL) _x000D_
Detection: HRP-conjugated Influenza A virus NP (nucleoprotein) antibody [HL1089] (GTX636247) (1 μg/mL)._x000D_

Please notice that GTX636247 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636318. Please contact us for custom HRP-conjugated antibody.

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of Influenza A virus (i.e., A/Kansas/2017 (H3N2); A/California/2009 (H1N1); A/Hong Kong/2671/2019 (H3N2)) and Influenza B virus (i.e., B/Sydney/3/2004; B/Victoria/02/1987) using antibodies as below._x000D__x000D_
Capture: Influenza A virus NP (nucleoprotein) antibody [HL1089] (GTX636247) (5 μg/mL) _x000D_
Detection: HRP-conjugated Influenza A virus NP (nucleoprotein) antibody [HL1103] (GTX636318) (1 μg/mL)._x000D_

Please notice that GTX636318 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636247. Please contact us for custom HRP-conjugated antibody.

Influenza A viral lysates (1 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.