Magic Red(TM) Cathepsin B Assay Kit

Artikelnummer: ICT-937
Artikelname: Magic Red(TM) Cathepsin B Assay Kit
Artikelnummer: ICT-937
Hersteller Artikelnummer: 937
Alternativnummer: ICT-937
Hersteller: ImmunoChemistry Technologies
Kategorie: Kits/Assays

Quantitate and monitor intracellular cathepsin-B activity over time in vitro. The Magic Red substrate in this assay fluoresces red upon cleavage by active cathepsin enzymes. Analyze the fluorescent signal using fluorescence microscopy or a fluorescence plate reader.


Background & Details

Elevated cathepsin enzyme activity in serum or the extracellular matrix can signify a number of pathological conditions.

ICT’s Magic Red assay kits are used by researchers seeking to quantitate and monitor cathepsin activity in cultured cells and tissues. This assay kit detects cathepsin-B activity. To use Magic Red, add the substrate directly to the cell culture media, incubate, and analyze. Because it is cell permeant, it easily penetrates the cell membrane and the membranes of the internal cellular organelles – no lysis or permeabilization steps are required. If cathepsin enzymes are active, the Magic Red substrate is cleaved and the cresyl violet fluorophore will become fluorescent upon excitation. As enzyme activity progresses and more Magic Red substrate is cleaved, the signal will intensify, allowing researchers to watch the color develop over time. Samples can be analyzed by fluorescence microscopy or with a fluorescence plate reader. Hoechst 33342 and Acridine Orange are included in the kit to detect nuclear morphology and lysosomal organelle structure, respectively.



Cathepsin B

Excitation / Emission

592 nm / 628 nm

Method of Analysis

Fluorescence Plate Reader, Fluorescence Microscope


Kit Contents

ICT-937 (25 Tests)

  • Magic Red Reagent (MR-RR2), 1 25-test vial
  • Hoechst 33342 Stain, 1 mL, (ICT-639)
  • Acridine Orange Stain, 0.5 mL, (ICT-6130)
  • Kit Manual
Magic Red® Cathepsin-B Assay Kit
Magic Red® Cathepsin-B Assay Kit (Components)