Peroxidase-conjugated AffiniPure Goat Anti-Human IgM, Fc5µ Fragment Specific (min X Bov Sr Prot)

Artikelnummer: JIM-109-035-129
Artikelname: Peroxidase-conjugated AffiniPure Goat Anti-Human IgM, Fc5µ Fragment Specific (min X Bov Sr Prot)
Artikelnummer: JIM-109-035-129
Hersteller Artikelnummer: 109-035-129
Alternativnummer: JIM-109-035-129
Hersteller: Jackson ImmunoResearch
Wirt: Goat
Kategorie: Antikörper
Spezies Reaktivität: Human
Konjugation: Horseradish Peroxidase

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc5µ portion of the human IgM heavy chain but not with human IgG, IgA, or the light chains of human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine serum proteins, but it may cross-react with IgM from other species.

 

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Klonalität: Polyclonal
Isotyp: Whole IgG
Puffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Reinheit: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Formulierung: Whole IgG
Application Verdünnung: 1:500 - 1:5,000 for immunohisto/cytochemistry 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substrates